在药品研发中，大家肯定遇到在复核USP标准的时候，总为不同的色谱柱而发愁，常常不能准确的确定USP规定的色谱柱，以及与我们常用色谱柱的关系，本文主要归纳一下USP色谱柱与我们常用色谱柱的关系。

 

L1和L8是美国药典(USP)规定的色谱柱编号，其实就是ODS柱和NH2柱。下面是USP规定的编号所对应的色谱柱类型。

 

L1：十八烷基键合多孔硅胶或无机氧化物微粒固定相，简称ODS柱

 

L2：30～50mm表面多孔薄壳型键合十八烷基固定相，简称C18柱

 

L3：多孔硅胶微粒，即一般的硅胶柱

 

L4：30～50mm表面多孔薄壳型硅胶柱

 

L5：30～50mm表面多孔薄壳型氧化铝柱

 

L6：30～50mm实心微球表面包覆磺化碳氟聚合物，强阳离子交换柱

 

L7：全多孔硅胶微粒键合C8官能团固定相，简称C8柱

 

L8：全多孔硅胶微粒键合非交联NH2固定相，简称NH2柱

 

L9：强酸性阳离子交换基团键合全多孔不规则形硅胶固定相，即SCX柱

 

L10：多孔硅胶微球键合氰基固定相（CN），简称CN柱

 

L11：键合苯基多孔硅胶微球固定相，简称苯基柱

 

L12：无孔微球键合季胺功能团的强阴离子交换柱

 

L13：三乙基硅烷化学键合全多孔硅胶微球固定相（C1），简称C1柱

 

L14：10mm硅胶化学键合强碱性季铵盐阴离子交换固定相，简称SAX柱

 

L15：已基硅烷化学键合全多孔硅胶微球固定相，简称C6柱

 

L16：二甲基硅烷化学键合全多孔硅胶微粒固定相 C2柱

 

L17：氢型磺化交联苯乙烯-二乙烯基苯共聚物,强阳离子交换柱

 

L18：3～10mm全多孔硅胶化学键合胺基（NH2）和氰基（CN）柱

 

L19：钙型磺化交联苯乙烯－二乙烯基苯共聚物，强阳离子交换柱

 

L20：二羟基丙烷基化学键合多孔硅胶微球固定相（Diol），简称二醇基柱

 

L21：刚性苯乙烯－二乙烯基苯共聚物微球填料柱

 

L22：带有磺酸基团的多孔苯乙烯阳离子交换柱

 

L23：带有季胺基团的聚甲基丙烯酸甲酯或聚丙烯酸酯多孔离子交换柱

 

L24：表面含有大量羟基的半刚性聚乙烯醇亲水凝胶柱

 

L25：聚甲基丙烯酸酯树脂交联羟基醚（表面含有残余羧基功能团）树脂。能分离分子量100～5000MW范围的水溶性中性、阳离子型及阴离子型聚合物（用聚氧乙烯测定）的固定相

 

L26：丁基硅烷化学键合全多孔硅胶微球固定相，即C4柱

 

L27：30～50mm的全多孔硅胶微粒

 

L28：多功能载体，100Å的高纯硅胶加以氨基键合以及C8反相键合的官能团

 

L29:氧化铝,反相键合,含碳量低,氧化铝基聚丁二稀小球,5mm，孔径80Å

 

L30:全多孔硅胶键合乙基硅烷固定相

 

L31:季胺基改性孔径2000Å的交联苯乙烯和二乙烯基苯(55%)强阴离子交换树脂

 

L32: L-脯氨酸铜配合物共价键合于不规则形硅胶微粒的配位体的交换手性色谱填料

 

L33:能够分离分子量4000～40000MW范围蛋白质分子的球形硅胶固定相, pH稳定性好

 

L34：铅型磺化交联苯乙烯－二乙烯基苯共聚物强阳离子交换树脂，9mm球形

 

L35：锆稳定的硅胶微球键合二醇基亲水分子单层固定相，孔径150Å

 

L36:5mm胺丙基硅胶键合L-苯基氨基乙酸－3,5二硝基苯甲酰

 

L37：适合分离分子量2000～40000MW的聚甲基丙烯酸酯凝胶

 

L38：水溶性甲基丙烯酸酯基质SEC色谱柱

 

L39：亲水全多孔聚羟基甲基丙烯酸酯色谱柱

 

L40：Tris 3,5－二甲基苯基氨基甲酸酯纤维素涂覆多孔硅胶微球

 

L41：球形硅胶表面固定α1酸糖蛋白固定相

 

L42: C8和C18硅烷化学键合多孔硅胶固定相

 

L43:硅胶微球键合五氟代苯基固定相

 

L44:多功能固定相,60 Å高纯硅胶基质键合磺酸阳离子交换功能团和C8反相功能团

 

L45: β-环糊精键合多孔硅胶微球

 

L46:季胺基改性苯乙烯-二乙烯基苯聚合物微球

 

L1 Octadecyl silane chemically bonded to porous silica orceramic.

 

L1 十八烷基键合硅烷化学键合于多孔硅胶或陶瓷微粒，3-10u。

 

L2 Octadecyl silane chemically bonded to silica gel of acontrolled surface porosity that has been bonded to a solid spherical core, 30to 50 μm in diameter.

 

L2 十八烷基键合硅烷化学键合于表面空隙率一定的键合在紧密球核上的硅胶，粒径30-50u。

 

L3 Porous silica particles, 5 to 10 μm in diameter.

 

L3 多孔硅胶颗粒，粒径5-10u。

 

L4 Silica gel of controlled surface porosity bonded to asolid spherical core,30 to 50 μm in diameter.

 

L4 表面空隙率一定的硅胶键合于紧密球核上，粒径30-50u。

 

L5 Alumina of controlled surface porosity bonded to asolid spherical core,30 to 50 μm in diameter.

 

L5 表面空隙率一定的氧化铝键合于紧密球核上，粒径30-50u。

 

L6 Strong cation-exchange packing: sulfonatedfluorocarbon polymer coated on a solid spherical core, 30 to 50 μm in diameter.

 

L6 强阳离子交换填料：磺化氟代烃聚合物涂渍于紧密球核上，粒径30-50u。

 

L7 Octyl silane chemically bonded to totally poroussilica particles, 1.5 to 10 μm in diameter.

 

L7 辛基硅烷化学键合于完全多孔硅胶担体，粒径10u。

 

L8 An essentially monomolecular layer ofaminopropyl-silane chemically bonded  to totally porous silica gelsupport, 3 to 10 μm in diameter.

 

L8 氨丙基硅烷单分子层化学键合于完全多孔硅胶担体，粒径10u。

 

L9 Irregular or spherical, totally porous silica gelhaving a chemically bonded, strongly acidic ation-exchange coating, 3 to 10 μmin diameter.

 

L9 10u不规则的完全多孔硅胶具有化学键合的强酸性阳离子交换涂层。

 

L10 Nitrile groups chemically bonded to porous silicaparticles, 3 to 10 μm in diameter.

 

L10 氰基团化学键合于多孔微粒硅，粒径3-10u。

 

L11 Phenyl groups chemically bonded to porous silicaparticles, 1.5 to 10 μm in diameter.

 

L11 苯基化学键合于多孔微粒硅，粒径3-10u。

 

L12 Strong anion-exchange packing made by chemically bondinga quaternary amine to a solid silica spherical core, 30 to 50 μm in diameter

 

L12 由四元胺化学键合于精密硅球核形成的强阴离子交换填料，粒径30-50u。

 

L13 Trimethylsilane chemically bonded to porous silicaparticles,3 to 10 μm in diameter.

 

L13 三甲基硅烷化学键合于完全多孔硅胶，粒径3-10u。

 

L14 Silica gel having a chemically bonded, strongly basicquaternary ammonium anion-exchange coating, 5 to 10 μm in diameter.

 

L14 具有强碱性四元胺阴离子交换涂层的10u硅胶化学键合相。

 

L15 Hexyl silane chemically bonded to totally poroussilica particles,3 to 10 μm in diameter.

 

L15 己基硅烷化学键合于完全多孔性微粒硅，粒径3-10u。

 

L16 Dimethyl silane chemically bonded to totally poroussilica particles, 5 to 10 μm in diameter.

 

L16 二甲基硅烷化学键合于完全多孔性微粒硅，粒径3-10u。

 

L17 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μmin diameter.

 

L17 由磺化的苯乙烯-二乙烯基苯交连共聚物组成的氢型阳离子交换树脂，粒径7-11u。

 

L18 Amino and cyano groups chemically bonded to poroussilica particles,3 to 10 μm in diameter.

 

L18 氨基和氰基化学键合于多孔微粒硅，粒径3-10u。

 

L19 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the calcium form, 9 μm indiameter.

 

L19 由磺化的苯乙烯-二乙烯基苯交连共聚物组成的钙型阳离子交换树脂，粒径7-11u。

 

L20 Dihydroxypropane groups chemically bonded to poroussilica particles, 5 to 10 μm in diameter.

 

L20 二甲氧基丙烷基化学键合于多孔硅胶微粒，粒径5-10u。

 

L21 Arigid, spherical styrene-divinylbenzene copolymer, 5 to 10 μm in diameter.

 

L21 刚性球形苯乙烯-二乙烯基苯共聚物，粒径5-10u。

 

L22 Acation exchange resin made of porous polystyrene gel with sulfonic acid groups,about 10 μm in size.

 

L22 由多孔聚苯乙烯凝胶和磺酸基组成的阳离子交换树脂，粒径约为10u。

 

L23 An anion exchange resin made of porouspolymethacrylate or polyacrylate gel with quaternary ammonium groups, about 10μm in size.

 

L23 由多孔聚甲基丙烯酸酯或聚丙烯酸酯凝胶及四元胺基组成的阴离子交换树脂，粒径约为10u。

 

L24 Asemi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxylgroups on the matrix surface, 32 to 63 μm in diameter.

 

L24 由乙烯基聚合物和基质表面的无数羟基组成的半刚性亲水硅胶，粒径32u-63u。

 

L25 Packing having the capacity to separate compoundswith a MW range from 100 to 5000 daltons (as determined by polyethylene oxide),applied to neutral, anionic, and cationic water-soluble polymers. Apolymethacrylate resin base,crosslinked with poly-hydroxylated ether (surfacecontained some residual carboxyl functional groups) was found suitable.

 

L25 能分离MW从100到5000Da化合物的填料（取决于聚环氧乙烷），应用于中性、阴离子和阳离子的水溶性聚合物。聚甲基丙烯酸酯作为树脂基质，与聚羟基化醚（表面含一些残余羧基官能团）进行交连。

L26 Butyl silane chemically bonded to totally poroussilica particles, 5 to 10 μm in diameter.

 

L27 Porous silica particles, 30 to 50 μm in diameter.

 

L28 Amultifunctional support, which consists of a high purity, 100 , sphericalsilica substrate that has been bonded with anionic (amine) functionality inaddition to a conventional reversed phase C8 functionality.

 

L29 Gamma alumina, reversed phase, low carbon percentageby weight, alumina-based polybutadiene spherical particles, 5 μm diameter witha pore diameter of 80 .

 

L30 Ethyl silane chemically bonded to a totally poroussilica particle, 3 to 10 μm in diameter.

 

L31 Astrong anion-exchange resin-quaternary amine bonded on latex particles attachedto a core of 8.5 μm macroporous particles having a pore size of 2000 Å

 

and consisting of ethylvinylbenzene cross-linked with 55% divinyl benzene.

 

L32 Achiral ligand-exchange packing- L-proline copper complex covalently bonded toirregularly shaped silica particles, 5 to 10 μm in diameter.

 

L33 Packing having the capacity to separate proteins of4,000 to 400,000 daltons. It is spherical, silica-based and processed toprovide pH stability.

 

L34 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene-divinylbenzene copolymer in the lead form, about 9 μm indiameter.

 

L35 Azirconium-stabilized spherical silica packing with a hydrophilic (diol-type)molecular monolayer bonded phase having a pore size of 150 .

 

L36 3,5-dinitrobenzoyl derivative of L-phenylglycinecovalently bonded to 5 μm aminopropyl silica.

 

L37 Polymethacrylate gel packing having the capacity toseparate proteins by molecular size over a range of 2,000 to 40,000D.

 

L38 Methacrylate-based size-exclusion packing forwater-soluble samples.

 

L39 Hydrophilic polyhydroxymethacrylate gel of totallyporous spherical resin.

 

L40 Cellulose tris-3,5-dimethylphenylcarbamate coatedporous silica particles,5 μm to 20 μm in diameter.

 

L41 Immobilized á-acid glycoprotein on spherical silicaparticles, 5 μm in diameter.

 

L42 Octylsilane and octadecylsilane groups chemicallybonded to porous silica particles,5 μm in diameter.

 

L43 Pentafluorophenyl groups chemically bonded to silicaparticles, 5 to 10 μm in diameter.

 

L44 Amultifunctional support, which consists of a high purity, 60 , spherical silicasubstrate that has been bonded with a cationic exchanger, sulfonic acidfunctionality in addition to a conventional reversed phase C8 functionality.

 

L45 Beta cyclodextrin bonded to porous silica particles,5 to 10 μm in diameter.

 

L46 Polystyrene/divinylbenzene substrate agglomeratedwith quaternary amine functionalized latex beads, 10 μm in diameter.

 

L47 High capacity anion-exchange microporous substrate,fully functionalized with a trimethylamine group, 8 μm in diameter.

 

L48 Sulfonated, cross-linked polystyrene with an outerlayer of submicron, porous,anion-exchange microbeads, 15 μm in diameter.

 

L49 Areversed-phase packing made by coating a thin layer of polybutadiene on tospherical porous zirconia particles, 3 to 10 μm in diameter.

 

L50 Multifunction resin with reversed-phase retention andstrong anion-exchange functionalities. The resin consists of ethylvinylbenzene,55 % cross-linked with

 

divinylbenzene copolymer, 3 to 15 μm in diameter, and asurface area of not less than 350 m2/g, substrate is coated with quaternary ammoniumfunctionalized latex particles consisting of styrene cross-linked withdivinylbenzene.

 

L51 Amylose tris-3,5-dimethylphenylcarbamate-coated,porous, spherical, silica particles,

 

5 to 10 μm in diameter.

 

L52 Astrong cation exchange resin made of porous silica with sulfopropyl groups, 5to 10 μm in diameter.

 

L53 Weak cation-exchange resin consisting ofethylvinylbenzene, 55 % cross-linked with

 

divinylbenzene copolymer, 3 to 15 μm diameter. Substrateis surface grafted with carboxylic acid and/or phosphoric acid functionalizedmonomers. Capacity not less than 500 μm in diameter.

 

L54 Asize exclusion medium made of covalent bonding of dextran to highlycross-linked porous agarose beads, about 13 μm in diameter.

 

L55 Astrong cation exchange resin made of porous silica coated withpolybutadiene-maleic acid copolymer, about 5 μm in diameter.

 

L56 Isopropyl silane chemically bonded to totally poroussilica particles, 3 to 10 μm in diameter.

 

L57 A chiral-recognitionprotein, ovomucoid, chemically bonded to silica particles, about 5 μm indiameter, with a pore size of 120 angstroms.

 

L58 Strong cation-exchange resin consisting of sulfonatedcross-linked styrene- divinylbenzene copolymer in the sodium form, about 7 to11 μm diameter.

 

L59 To separate proteins by molecular weight over therange of 10 to 500 kDa. Spherical 10 μm, silica-based, and processed to providehydrophilic characteristics and pH stability.

 

L60 Spherical, porous silica gel, 3 to 10 μm in diameter,surface has been covalently modified with palmitamidopropyl groups andendcapped.

 

L61 Hydroxide-selective, strong anion-exchange resinconsisting of a highly cross-linked core of 13 μm microporous particles, poresize less than 10 , and consisting of ethylvinylbenzene cross-linked with 55 %divinylbenzene with a latex coating

 

composed of 85 nm diameter microbeads bonded with alkanolquarternary ammonium ions (6 %).

 

L62 C30silane bonded phase on a fully porous spherical silica, 3 to 15 μm in diameter.